Table 7 Oligonucleotid primers used for detection of virulence factors, serogroups and antibiotic resistance genes in STEC strains isolated from calves with diarrhea
Primer name | Sequence (5’-3’) | Size of product (bp) | Target gene | References |
|---|---|---|---|---|
Hly-F | GAGCGAGCTAAGCAGCTTG | 889 | hlyEHEC | [6] |
Hly-R | CCTGCTCCAGAATAAACCACA | |||
Stx1-F | CAGTTAATGTGGTGGCGAAGG | 348 | Stx1 | [53] |
Stx1-R | CACCAGACAATGTAACCGCTG | |||
Stx2-F | ATCCTATTCCCGGGAGTTTACG | 584 | Stx2 | [53] |
Stx2-R | GCGTCATCGTATACACAGGAGC | |||
Eae-F | TGCGGCACAACAGGCGGCGA | 629 | eae | [6] |
Eae-R | CGGTCGCCGCACCAGGATTC | |||
Cnf1-F | GGGGGAAGTACAGAAGAATTA | 1111 | cnf1 | [21] |
Cnf1-R | TTGCCGTCCACTCTCTCACCAGT | |||
Cnf2-F | TATCATACGGCAGGAGGAAGCACC | 1240 | cnf2 | [21] |
Cnf2-R | GTCACAATAGACAATAATTTTCCG | |||
Cdt3-F | GAAAATAAATGGAATATAAATGTCCG | 555 | cdt-III | [21] |
Cdt3-R | TTTGTGTCGGTGCAGCAGGGAAAA | |||
F17c-F | GCAGGAACCGCTCCCTTGGC | 416 | f17c | [23] |
F17c-R | CAACTAACGGGATGTACAGTTTC | |||
LT-F | GCACACGGAGCTCCTCAGTC | 218 | ltII | [53] |
LT-R | TCCTTCATCCTTTCAATGGCTTT | |||
ST-F | AGGAACGTACATCATTGCCC | 521 | st | [54] |
ST-R | CAAAGCATGCTCCAGCACTA | |||
O26-F | CAG AAT GGT TAT GCT ACT GT | 423 | wzx | [55] |
O26-R | CTT ACA TTT GTT TTC GGC ATC | |||
O103-F | TTGGAGCGTTAACTGGACCT | 321 | wzx | [55] |
O103-R | GCTCCCGAGCACGTATAAG | |||
O111-F | TAG AGA AAT TAT CAA GTT AGT TCC | 406 | wzx | [55] |
O111-R | ATA GTT ATG AAC ATC TTG TTT AGC | |||
O145-F | CCATCAACAGATTTAGGAGTG | 609 | wzx | [55] |
O145-R | TTTCTACCGCGAATCTATC | |||
O157-F | CGG ACA TCC ATG TGA TAT GG | 259 | wzx | [55] |
O157-R | TTG CCT ATG TAC AGC TAA TCC | |||
O45-F | CCG GGT TTC GAT TTG TGA AGG TTG | 527 | wzx1 | [56] |
O45-R | CAC AAC AGC CAC TAC TAG GCA GAA | |||
O91-F | GCTGACCTTCATGATCTGTTGA | 291 | gnd | [57] |
O91-R | TAATTTAACCCGTAGAATCGCTGC | |||
O113-F | GGGTTAGATGGAGCGCTATTGAGA | 771 | wzx | [12] |
O113-R | AGGTCACCCTCTGAATTATGGCAG | |||
O121-F | TGGCTAGTGGCATTCTGATG | 322 | wzx | [58] |
O121-R | TGATACTTTAGCCGCCCTTG | |||
O128-F | GCTTTCTGCCGATATTTGGC | Â | galF | [59] |
O128-R | CCGACGGACTGATGCCGGTGATT | |||
aadA1-F | TATCCAGCTAAGCGCGAACT | 447 | Streptomycin resistance | [60] |
aadA1-R | ATTTGCCGACTACCTTGGTC | |||
tetA- F | GGTTCACTCGAACGACGTCA | 577 | Tetracycline resistance | [60] |
tetA- R | CTGTCCGACAAGTTGCATGA | |||
tetB- F | CCTCAGCTTCTCAACGCGTG | 634 | Tetracycline resistance | [60] |
tetB- R | GCACCTTGCTGATGACTCTT | |||
dfrA1- F | GGAGTGCCAAAGGTGAACAGC | 367 | Trimethoprim resistance | [61] |
dfrA1- R | GAGGCGAAGTCTTGGGTAAAAAC | |||
Qnr- F | GGGTATGGATATTATTGATAAAG | 670 | Fluoroquinolone resistance | [62] |
Qnr- R | CTAATCCGGCAGCACTATTTA | |||
aac [3] -IV- F | CTTCAGGATGGCAAGTTGGT | 286 | Gentamicin resistance | [63] |
aac [3] -IV- R | (TCATCTCGTTCTCCGCTCAT | |||
Sul1- F | TTCGGCATTCTGAATCTCAC | 822 | Sulfonamide resistance | [63] |
Sul1- R | ATGATCTAACCCTCGGTCTC | |||
blaSHV- F | TCGCCTGTGTATTATCTCCC | 768 | Cephalothin resistance | [63] |
blaSHV- R | CGCAGATAAATCACCACAATG | |||
CITM- F | TGGCCAGAACTGACAGGCAAA | 462 | Ampicillin resistance | [63] |
CITM- R | TTTCTCCTGAACGTGGCTGGC | |||
ereA- F | GCCGGTGCTCATGAACTTGAG | 419 | Erythromycin resistance | [63] |
ereA- R | CGACTCTATTCGATCAGAGGC | |||
cat1- F | AGTTGCTCAATGTACCTATAACC | 547 | Chloramphenicol resistance | [63] |
cat1- R | TTGTAATTCATTAAGCATTCTGCC | |||
cmlA-F | CCGCCACGGTGTTGTTGTTATC | 698 | Chloramphenicol resistance | [63] |
cmlA-R | CACCTTGCCTGCCCATCATTAG | Â | Â | Â |