TY - JOUR AU - Traipe-Castro, Leonidas AU - Salinas-Toro, Daniela AU - López, Daniela AU - Zanolli, Mario AU - Srur, Miguel AU - Valenzuela, Felipe AU - Cáceres, Aníbal AU - Toledo-Araya, Héctor AU - López-Solís, Remigio PY - 2014 DA - 2014/06/04 TI - Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment JO - Biological Research SP - 25 VL - 47 IS - 1 AB - Fern-like crystalloids form when a microvolume of tear is allowed to dry out at ambient conditions on a glass surface. Presence of crystalloids in tear “microdesiccates” is used to evaluate patients with Dry-Eye disease. This study aims to examine morphologically the desiccation process of normal tear fluid and to identify changes associated with accelerated tear evaporation. Tear microdesiccates from healthy (Non-Dry Eye) and Dry Eye subjects were produced at ambient conditions. Microdesiccate formation was monitored continuously by dark-field video microscopy. Additionally, accelerated desiccation of tear samples from healthy subjects was conducted under controlled experimental conditions. Particular morphological domains of tear microdesiccates and their progressive appearance during desiccation were compared. SN - 0717-6287 UR - https://doi.org/10.1186/0717-6287-47-25 DO - 10.1186/0717-6287-47-25 ID - Traipe-Castro2014 ER -