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Table 1 Precision of the SYBR Green Real time PCR assay determined in this study

From: A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting

Reference concentration (copies/ml) Measured concentration (copies/ml) Standard deviation (SD) Coefficient of variation (CV)
52 50 1.65 0.0325
124 120 3.06 0.0251
344 345 0.47 0.0014
871 863 5.42 0.0063
1,235 1,250 10.14 0.0082
16,383 16,358 17.68 0.0011
267,816 267,098 507.70 0.0019
31,293 31,299 4.48 0.0001
541 534 4.71 0.0088
1,476 1,466 6.60 0.0045
4,572 4,555 12.26 0.0027
2571,210 2569,050 1,526.88 0.0006
76 89 9.43 0.1145
43,110 43,135 18.15 0.0004
5,447 5,414 23.10 0.0043
21,312 21,393 57.28 0.0027
96 94 1.65 0.0174
635,469 635,592 86.97 0.0001
626 620 4.48 0.0072
54,886 54,853 23.81 0.0004
8,742 8,781 27.58 0.0031
917,838 917,648 133.88 0.0001
74,511 74,552 29.23 0.0004
944 965 15.32 0.0161
9,648 9,984 237.82 0.0242
  1. The standard deviation and the coefficient of variation were determined using data from 10 independent experiments.
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