Figure 2
From: Methanol extract from Vietnamese Caesalpinia sappan induces apoptosis in HeLa cells
The increment of caspase-3 activity in HeLa cells in vitro. After 12, 24 and 48 h incubation with MECS, HeLa cells lysates were incubated at 37°C with caspase-3 substrate (Ac-DEVD-AFC) for 1 h. The fluorescence intensity of the cell lysates was measured to determine the caspase-3 activity. The blank group was used as 0.1% DMSO-treated cells; Camptothecin (2.5 μg/mL) was used as positive control. Data are presented as the mean ± S.D. of results from three independent experiments (* P < 0.05 vs. control).