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Figure 6 | Biological Research

Figure 6

From: First report of in vitro selection of RNA aptamers targeted to recombinant Loxosceles laeta spider toxins

Figure 6

Synthesis of random sequence template DNA (s0) by PCR. A. Amplification of oligonucleotide TG-503 (96 nt) by PCR with primers TG-504 (46 nt) and TG-505 (27 nt); the template has a 60 nt central region of random sequence. The resulting s0 DNA (133 bp) has a promoter for T7 RNA polymerase, which allows the generation of RNA by in vitro transcription, and recognition sites for restriction enzymes Hin dIII and Xba I, which allow cloning for the analysis of individual sequences. B. Nucleotide sequence of s0 DNA.

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Biological Research

ISSN: 0717-6287

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