Figure 2

Effects of egg extract on voltage-gated sodium channels in rat DRG neurons. Legend: Patch-clamp technique was used to measure the sodium currents of rat DRG neurons (n = 5). (A) TTX-R Na⁺ currents were induced by a 50-ms step depolarization to −10 mV from a holding potential of −80 mV every 5 seconds in small DRG neurons in the presence and absence of 100 μg/mL egg extract. (B) TTX-S Na⁺ currents were induced by a 50-ms step depolarization to −10 mV from a holding potential of −80 mV every 5 seconds in large DRG neurons in the presence and absence of 100 μg/mL egg extract. (C) Current–voltage curves of TTX-R Na⁺ channels in the presence and absence of 100 μg/mL egg exact. (D) Steady-state activation curves of TTX-R Na⁺ channels in the presence and absence of 100 μg/mL egg extract. G was normalized to the peak membrane conductance (Gmax ) at +30 mV. The curve was fitted with the equation G = Gmax /[1 + exp (V0.5-V)/k].